Journal: Disease Models & Mechanisms
Article Title: Vemurafenib improves muscle histopathology in a mouse model of LAMA2 -related congenital muscular dystrophy
doi: 10.1242/dmm.049916
Figure Lengend Snippet: Vemurafenib inhibited mTOR and p70S6K activation in dy W−/− mice. Levels of (A) phosphorylated ERK1/2 (pT202/Y204), (B) phosphorylated STAT3 (pY705), (C) phosphorylated NFκB p65 (pS536), (D) phosphorylated JNK1/2 (pT183/Y185), (E) phosphorylated mTOR (pS2448) and (F) phosphorylated p70S6K (pT389) in protein extracts of gastrocnemius muscle from WT mice, vehicle-treated dy W−/− mice and vemurafenib-treated dy W−/− mice. One-way ANOVA with uncorrected Fisher's LSD test was performed for the data that followed the normal distribution [ERK1/2 (pT202/Y204), mTOR (pS2448) and NFκB p65 (pS536)]. Kruskal–Wallis test was performed for the data that did not follow the normal distribution [STAT3 (pY705), JNK1/2 (pT183/Y185) and p70S6K (pT389)]. All data are represented by statistical significance of mean±s.e.m. ( n =10 for all groups). ns, not significant; * P <0.05; ** P <0.01; *** P <0.001; **** P <0.0001.
Article Snippet: Enzyme-linked immunosorbent assay (ELISA) kits were used to measure levels of active caspase 3 (MyBioSource, MBS7210856), TGF-β1 (Abcam, ab119557), phosphorylated SMAD3 (pS423/S425) (Abcam, 186038), phosphorylated mTOR (pS2448) (RayBiotech, PEL-mTOR-S2448), phosphorylated p70S6K (pT389) (Abcam, ab176651), phosphorylated ERK1/2 (pT202/Y204) (Abcam, ab176640), phosphorylated STAT3 (pY705) (Invitrogen, KHO0481), phosphorylated NFκB p65 (pS536) (Abcam, ab176647), phosphorylated JNK1/2 (pT183/Y185) (Abcam, ab176645), atrogin1 (LSBio, LS-F35338), MuRF1 (MyBioSource, MBS2502946), beclin-1 (LSBio, LS-F35824) and p62 (MyBioSource, MBS039475) in the gastrocnemius protein homogenate, according to the manufacturer's instructions.
Techniques: Activation Assay
Bio-Rad is a verified supplier 
![Vemurafenib inhibited mTOR and p70S6K activation in dy W−/− mice. Levels of (A) phosphorylated ERK1/2 (pT202/Y204), (B) phosphorylated STAT3 (pY705), (C) phosphorylated NFκB p65 (pS536), (D) phosphorylated <t>JNK1/2</t> <t>(pT183/Y185),</t> (E) phosphorylated mTOR (pS2448) and (F) phosphorylated p70S6K (pT389) in protein extracts of gastrocnemius muscle from WT mice, vehicle-treated dy W−/− mice and vemurafenib-treated dy W−/− mice. One-way ANOVA with uncorrected Fisher's LSD test was performed for the data that followed the normal distribution [ERK1/2 (pT202/Y204), mTOR (pS2448) and NFκB p65 (pS536)]. Kruskal–Wallis test was performed for the data that did not follow the normal distribution [STAT3 (pY705), JNK1/2 (pT183/Y185) and p70S6K (pT389)]. All data are represented by statistical significance of mean±s.e.m. ( n =10 for all groups). ns, not significant; * P <0.05; ** P <0.01; *** P <0.001; **** P <0.0001.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_4677/pmc10184677/pmc10184677__dmm-16-049916-g4.jpg)